This product is provided as three 5 nmol vials (15 nmol) or 2x three 5 nmol vials (30 nmol) of lyophilized siRNA oligo duplexes. Each vial contains slightly different sequences to ensure full knockout of the gene. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. The siRNA oligos are also chemically modified (2"-OMe) for increased stability and enhanced knockdown in vitro and in vivo.
| Target | ABCA4 |
| Reactivity | Human |
| Tested Applications | RNAi |
| Host | Synthetic |
| Form | Lyophilized |
| Purity | > 97% |
| Quality Control | Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. |
| Storage | Shipped at 4 °C. Store at -20 °C for up to one year. |
| UniProt Primary AC | P78363 (UniProt, ExPASy) |
| Gene Symbol | ABCA4 |
| GeneID | 24 |
| Specificity | ABCA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression. |
| Directions for use | 1. Transfect with 100 nM siRNA 48 to 72 hours prior to cell lysis.2. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.3. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. Each vial is suitable for 250 transfections in a 24 well plate (20 pmol for each well). |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
Abbexa是一家致力于为生命科学研究、药物研发及生物技术公司提供优质抗体、蛋白及检测试剂盒等产品的高科技生物技术公司。基于剑桥大学研发技术优势，Abbexa提供包括一抗、二抗、纯化蛋白、ELISA试剂盒、各种酶类及其它多种试剂与研究工具的大量生命科学研究产品。
目前Abbexa的产品涵盖单克隆抗体、多克隆抗体、纯化蛋白质及生物试剂，此外，为满足研发人员特殊的实验需要，Abbexa还提供抗体/多肽定制服务。严格的质控标准保证了Abbex产品的高性能，如您对产品不满意，Abbexa将直接提供退／换货服务
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